ROBINSON1

3D Optical Near-field Ptychography

Type

Experimental

#students

1

Orientation

Why is the scientific problem of interest at all?  .

 

How

How is the research going to shed light on the given problem?.

The goal is to determine to Ôoptical volumeÕ filled by each of the 23 different human chromosomes and compare with probe-based labelling methods, such as M-FISH.

  

What

What is the specific thing that the student will do, and how does it fit inside the overall project?

This project will involve setting up a ptychography instrument in a laser lab, consisting of a small laser, sample XYZ scan stage and a CMOS detector.  The ptychography method scans a small coherent spot over the sample and collects the diffraction pattern. An algorithm called Òsingle shotÓ PIE is then used to invert the diffraction to an image.  The project will be to develop a near-field version of the method using lenses to apply a modulated structure to the scanned probe.

 

If time permits, the student will then learn how to make chromosome ÔspreadsÕ and analyse their structure by ptychography.

Special Knowlegde

Note: this project is not based on UCL campus.  The experiments will take place at Harwell (near Oxford) requiring significant time spent travelling

 

Supervisor

Prof Ian Robinson i.robinson@ucl.ac.uk